Field-deployable and/or High Throughput Genetic Testing

Picture of Gene-Z

My group has also been engaged in developing molecular tools and platforms to screen for markers in field-deployable formats and high throughput screening of important functional genes (e.g., antimicrobial resistance, oxygenases, dehalogenase, etc.). Key tools that my group has developed include i) Gene-Z - a hand-held device for nucleic acids testing (Stedtfeld at al., 2012), ii) an antimicrobial resistance gene screening chip containing approx. 500 AMR genes based on Wafergen's SmartChip platform (Zhu et al., 2013, Johnson et al.,2016, Stedtfeld et al., 2018), and iii) a direct amplification approach to allow simplified field-based amplification systems (Williams et al., 2017). The OHRB chip being used as part of the work proposed here is based on the expertise accumulated as part of the above research.

Selected Publications

  • Stedtfeld, Robert D.; Tourlousse, Dieter M.; Seyrig, Gregoire; Stedtfeld, Tiffany M.; Kronlein, Maggie; Price, Scott; Ahmad, Farhan; Gulari, Erdogan; Tiedje, James M.; Hashsham, Syed A. Gene-Z: a device for point of care genetic testing using a smartphone. Lab on Chip, 12(8): 1454-1462, 2012.
  • Zhu, YG; Johnson, TA; Su, JQ; Qiao, M; Guo, GX; Stedtfeld, RD; Hashsham, SA; Tiedje, JM. Diverse and abundant antibiotic resistance genes in Chinese swine farms. Proceedings of the National Academy of Sciences of the United States of America. 110(9): 3435-3440, Feb 2013.
  • Williams, M. R., R. D. Stedtfeld, H. Waseem, T. Stedtfeld, B. Upham, W. Khalife, B. Etchebarne, M. Hughes, J. M. Tiedje and S. A. Hashsham. Implications of direct amplification for measuring antimicrobial resistance using point-of-care devices. Analytical Methods. DOI: 10.1039/c6ay03405e. January 2017.
  • Stedtfeld, Robert; Guo, Xueping; Stedtfeld, Tiffany; Sheng, Hongjie; Williams, Maggie; Hauschild, Kristin; Gunturu, Santosh; Tift, Leo; Wang, Fang; Howe, Adina; Chai, Benli; Daqiang, Yin; Cole, James; Tiedje, James; Hashsham, Syed. Primer set 2.0 for highly parallel qPCR array targeting antibiotic resistance genes and mobile genetic elements. FEMS Microbiology Ecology. 2018, Vol. 94, No. 9.