Genomics

Enabling Technologies for Organohalide Bioremediation

My interest in developing technologies for enhancement of rate, isolation of novel OHRB, rapid and economical measurement of biomarkers under field conditions, and high throughput molecular characterization for delineation of the populations present and active started with my doctoral work and remains one of the key areas of my research interests. Cbl-mediated enhancement in rate of transformation including adsorption of Cbl to matrices relevant to remediation (Hashsham et al., 1995, Hashsham and Freedman, 1999), isolation of OHRB (Sun et al., 2002), measuring Dehalococcoides abundance in the field using rapid and easy to use molecular tools (Stedtfeld et al., 2014), and genomics and molecular insights into microbial populations involved in remediation (Chai et al., 2016) are some of the key examples. More recent work involves metabolomics, proteomics (in collaboration with Pacific Northwest National lab), and metagenomic tools.

Selected Publicatons

  • Hashsham, S.A., R. Scholze, and D.L. Freedman. Cobalamin-enhanced anaerobic biotransformation of carbon tetrachloride. Environmental Science & Technology. 29(11):2856-2863, 1995.
  • Hashsham, S.A. and D.L. Freedman. Enhanced biotransformation of carbon tetrachloride by Acetobacterium woodii upon addition of hydroxocobalamin and fructose. Applied and Environmental Microbiology. 65(10): 4537-4542, 1999.
  • Sun, B., B.M. Griffin, R.K. Kehrer, S.A. Hashsham, and J.M. Tiedje. Microbial reductive dechlorination of 1,1,1-trichloroethane to chloroethane, Science. 298: 1023, 2002.
  • Robert Stedtfeld, Tiffany M Stedtfeld, Maggie Kronlein, Gregoire Seyrig, Robert J Steffan, Alison Cupples, Syed A Hashsham. DNA Extraction-free Quantification of Dehalococcoides spp. in Groundwater using a Hand-Held Device. Environmental Science & Technology. 10/2014; DOI: 10.1021/es503472h.
  • Chai, BL; Tsoi, TV; Iwai, S; Liu, C; Fish, JA; Gu, C; Johnson, TA; Zylstra, G; Teppen, BJ; Li, H; Hashsham, SA; Boyd, SA; Cole, JR; Tiedje, JM. Sphingomonas wittichii Strain RW1 Genome-Wide Gene Expression Shifts in Response to Dioxins and Clay. PLoS ONE. 11(6). Article Number: e0157008. DOI: 10.1371/journal.pone.0157008. 2016.