Project 4: Gut Microbiome-Host-Toxicant Interactions

Gut Microbiome-Host Interactions in Response to TCDD Exposure: The aims of this project are: 1): Determine the effect of TCDD exposure on the role of segmented filamentous bacteria (SFB) and cluster IV and XIVa Clostridia in modulating Treg/Th17 balance and determine the unique functional gene repertoire of SFB. 2): Evaluate the transcriptome response of SFB and cluster IV and XIVa Clostridia in response to TCDD, 2,3,4,7,9-PeCDF, and 2,3,7,8-TCDF exposure. and 3): Examine the effects of TCDD and related compounds on the metabolism of choline by the host and the gut microbiome, and the potential roles in the development of fatty liver and altering urinary trimethylamine (TMA)/ trimethylamine-oxide (TMAO) ratios.

Selected Publicatons

• Stedtfeld RD, Chai Benli, Crawford RB, Stedtfeld TM, Williams MR, Shao X, Kuwahara T, Cole JR, Kaminski NE, Tiedje JM, and S. Hashsham. "Modulatory influence of segmented filamentous bacteria on transcriptomic response of gnotobiotic mice exposed to TCDD ". Frontiers in Microbiology. Vol. 8. PMID: 28936204. (2017).
• Stedtfeld RD, Stedtfeld TM, Fader K, Williams MR, Bhaduri P, Quensen J, Zacharewski T, Tiedje JM, and S. Hashsham. "TCDD influences reservoir of antibiotic resistance genes in murine gut microbiome". FEMS Microbiology Ecology. Vol. 93. No. 5. PMID: 28475713. (2017).
• Williams MR, Stedtfeld RD, Tiedje JM, Hashsham, SA. "MicroRNAs-based inter-domain communication between the host and members of the gut microbiome ". Frontiers in Microbiology. Vol. 8. PMID: 29021788. (2017).
• Stedtfeld RD, Sallach JB, Crawford RB, Stedtfeld TM, Williams MR, Waseem H, Johnston C, Li H, Boyd SA, Kaminski NE, Teppen BJ, Tiedje JM, and S Hashsham. "TCDD administered on activated carbon eliminates bioavailability and subsequent shifts to a key murine gut commensal ". Applied Microbiology and Biotechnology. Vol. 101. No. 19. pp. 7409-7415. PMID: 28812142. (2017).