Publication Highlights

Deep sequencing methods for protein engineering and design

This reviews the pairing of next generation sequencing technologies with high throughput screens for studying and designing protein-protein interactions and enzymatic functions.

Wrenbeck EE, Faber M, Whitehead TA (2017) “Deep sequencing methods for protein engineering and design”, Current Opinion in Structural Biology 45:36-44

Rapid fine conformational epitope mapping using comprehensive mutagenesis and deep sequencing

By combining fluorescence activated cell sorting, yeast surface display, and next generation sequencing we present and validate an experimental pipeline that determines conformational epitopes. We determine the epitopes for multiple antigen-antibody interactions and compare our findings with previously reported X-ray crystallography data.

Published Manuscripts



32. Wang X, Stapleton JA, Klesmith JR, Hewlett E, Whitehead TA, Maynard J (2017) “Fine epitope mapping of two antibodies neutralizing the Bordetella adenylate cyclase toxin”, Biochemistry in press doi: 10.1021/acs.biochem.6b01163

31. Klesmith JR, Bacik JP, Wrenbeck EE, Michalczyk R, Whitehead TA (2017) “Trade-offs between enzyme fitness and solubility illuminated by deep mutational scanning”, PNAS in press doi: 10.1073/pnas.1614437114