Deep sequencing methods for protein engineering and design
This reviews the pairing of next generation sequencing technologies with high throughput screens for studying and designing protein-protein interactions and enzymatic functions.
Wrenbeck EE, Faber M, Whitehead TA (2017) “Deep sequencing methods for protein engineering and design”, Current Opinion in Structural Biology 45:36-44
Rapid fine conformational epitope mapping using comprehensive mutagenesis and deep sequencing
By combining fluorescence activated cell sorting, yeast surface display, and next generation sequencing we present and validate an experimental pipeline that determines conformational epitopes. We determine the epitopes for multiple antigen-antibody interactions and compare our findings with previously reported X-ray crystallography data.