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BIOTECHNOLOGY: 

   MICRO-ARRAYS OF BIO-MATERIALS (e.g., for HIGH THROUGHPUT SCREENING)

   

In the field of biotechnology, we would like to utilize both the self-assembly and the layer-by layer techniques, including surface tuning and patterning, to develop new devices and sensors, especially in high throughput screening test, in which a lot of well-arrayed bio-materials on limited surface is key to analyze many different cases (e.g., mutants) by a random mutation process.  The ability to make micro arrays of small to large molecules on surfaces can directly be adjusted to prepare biomaterials arrays on surfaces, such as DNA, protein, and cells.  This is why the technique has attracted a great interest for their important industrial applications. With the knowledge obtained in micro processing of self-assembly technique, using thiols and silanes, polyelectrolytes, colloidal & metal nano- particles and coatings, various functional and topological patterned surfaces can be developed, to be good model substrates for the potential application of biomaterials array on surface.  For example, in order to study protein function, it is necessary to immobilize the proteins in a way that preserves their folded conformations. In addition, it is critical to minimize nonspecific binding of other proteins to the surface in subsequent steps. To accomplish these goals, we can use a variety of chemically derivatized slides, which are micro-patterned on surfaces.  Because typical proteins display many lysines on their surface as well as the generally more reactive alpha-amine at their amino termini, they can attach to the slide in a variety of orientations, permitting different sides of the protein to interact with other proteins or small molecules in solution. Biomaterials micro-arrays can be used to screen rapidly for biomaterial-biomaterial interactions, and can also be used to detect enzyme-substrate interactions. Finally, those microarrays can be used to identify the biomaterials targets of biologically active small molecules.

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Copyright © 2002 Ilsoon Lee All rights reserved.

 

 


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