Abstracts*

* Accompanied by poster presentation and published in conference proceedings

  1. Dieter M Tourlousse, Ahsan Munir, Robert D Stedtfeld,  Farhan Ahmad, Tiffany Stedtfeld, Amanda B Herzog, Jean Marie Rouillard, Erdogan Gulari, James M Tiedje, and Syed A Hashsham. An in situ synthesized microfluidic DNA biochip to detect 20 waterborne pathogens. Nanotech 2007 Conference, Santa Clara, CA, May 20-24, 2007.
  2. Robert Stedtfeld, Dieter Tourlousse, Farhan Ahmad, Vidya Srinivasan, Tiffany Stedtfeld, Benli Chai, James R. Cole, Erdogan Gulari, James Tiedje, and Syed A. Hashsham. Parallel Detection and Quantification of Pathogens using DNA Biochips, On-Chip PCR, and Hand-Held Devices. COST B28 Meeting, December 10-12, Vienna, Austria, 2007.
  3. E. Cardenas, J.R. Cole, A.S. Kulam-Syed-Mohideen, Q. Wang, B. Chai, B.Upham, S. Hashsham, J. Trosko, and N.E. Kaminski. The MSU SBRP Molecular Biology Tools Repository. NIEHS SBRP Superfund Symposium, Research Triangle Park, N.C., November 2007.
  4. Tiffany M. Stedtfeld, Robert D. Stedtfeld, Sam W. Baushke, Sarah M. Miller, Erdogan Gulari, James M. Tiedje, Syed A. Hashsham. Use of Multiple Genetic Markers as Indicators for Fecal Contamination in Environmental Water Sources. 107th General Meeting of the American Society for Microbiology, Toronto, Canada, Ontario, May 21-25, 2007.
  5. Hashmi, I., D. M. Tourlousse, R. D. Stedtfeld, S. W. Baushke, T. M. Stedtfeld, S. M. Miller, J. M. Tiedje, S. A. Hashsham; High Throughput Real-Time PCR to Monitor Synthrophic and Methanogenic Population Dynamics in Anaerobic Bioreactor Communities. 107th General Meeting of the American Society for Microbiology, Toronto, Canada, Ontario, May 21-25, 2007.
  6. Yu Yang, Syed A Hashsham, and James M Tiedje.. Characterization of Dye-doped Nanoparticles and Their Application on Quantitation and Viability Measurement. 107th General Meeting of the American Society for Microbiology, Toronto, Canada, Ontario, May 21-25, 2007.
  7. Alok K. Pandey, Amanda B. Herzog, Joan B. Rose, Syed A. Hashsham. Potential of Quantum Dots as Surrogates for Microbial Pathogens and Evaluation of Their Genotoxicity. 107th General Meeting of the American Society for Microbiology, Toronto, Canada, Ontario, May 21-25, 2007.
  8. Farhan Ahmad, Dieter M Tourlousse, Sarah M Miller, Robert D Stedtfeld, Erdogan Gulari, James M Tiedje, Syed A Hashsham. An in situsynthesized 50-mer oligonucleotide microfluidic biochip for the detection of virulence genes of waterborne pathogens. 107th General Meeting of the American Society for Microbiology, Toronto, Canada, Ontario, May 21-25, 2007.
  9. V. Srinivasan, X. Yu, D. M. Tourlousse, R. D. Stedtfeld, S. M. Miller, T. Pham, J. M. Rouillard, J. M. Tiedje, E. Gulari, S. A. Hashsham. Cost Effective Detection of Multiple Waterborne Pathogens with Gold Nanoparticles and Silver Enhancement on a Microarray Using a Flatbed Scanner. 107th General Meeting of the American Society for Microbiology, Toronto, Canada, Ontario, May 21-25, 2007.
  10. Amanda B. Herzog, Alok Pandey, Tomoyuki Shibata, Joan B. Rose, Syed A. Hashsham. Implications of Detection Limit of Various Methods of Bacillus anthracis in Computing Risk to Human Health. 107th General Meeting of the American Society for Microbiology, Toronto, Canada, Ontario, May 21-25, 2007.
  11. Dieter Tourlousse, Farhan Ahmad, Sarah Miller, Erdogan Gulari, James Tiedje, and Syed Hashsham Optimization of Gene-Specific Helicase Dependent-Amplification for Hand-held Pathogen Detection Devices.107th General Meeting of the American Society for Microbiology, Toronto, Canada, Ontario, May 21-25, 2007.
  12. R. D. Stedtfeld, S. W. Baushke, S.M. Miller, E. Gulari, J. M. Tiedje, S. A. Hashsham. Development of PCR-based tools for pathogen detection in environmental waters: minimizing validation by targeting multiple virulence and marker genes. 107th General Meeting of the American Society for Microbiology, Toronto, Canada, Ontario, May 21-25, 2007.
  13. Aijie Wang, Yingjun Shi, S. Hashsham, J. Tiedje, Nanqi Ren, Zhenguo Yu, Ying Liu, Yangguo Zhao. Improvement Of Fermentative Hydrogen Production Capacity From Cellulose via Bacterial Interspecific Cooperation.  107th General Meeting of the American Society for Microbiology, Toronto, Canada, May 21-25, 2007.
  14. Ahsan Munir, Sarah M Miller, Alok Dhawan, Robert D Stedtfeld, Jean Marie Rouillard, Erdogan Gulari, James M Tiedje, and Syed A Hashsham. Hybridization Behavior of Vancomycin Resistance Genes in a Flow-Through DNA Biochip using a Computational Approach. 107th General Meeting of the American Society for Microbiology, Toronto, Canada, Ontario, May 21-25, 2007.
  15. Alok Dhawan, Julian S. Taurozzi, Alok K. Pandey, Wenqian Shan, Sarah M. Miller, Syed A. Hashsham, and Volodymyr V. Tarabara. 2006. Stable Colloidal Dispersions of C60 Fullerenes in Water: Evidence for Genotoxicity. The 2006 AICHE Annual Meeting, November 12-17, 2006, San Francisco.
  16. R. D. Stedtfeld, S. W. Baushke, S. Miller, J. M. Tiedje, S. A. Hashsham. Microchamber Biochip for Quantitative Detection of Human Pathogens in Environmental Samples. 106th General Meeting of the American Society for Microbiology, Orlando, Florida, May 21-25, 2006.
  17. Dieter M Tourlousse, Sarah M Miller, Robert D Stedtfeld, Amanda B Herzog, Samuel W Baushke, Lukas M Wick, Jean Marie Rouillard, Erdogan Gulari, James M Tiedje, and Syed A Hashsham. Validation of an in situ synthesized oligonucleotide biochip for parallel detection of 12 waterborne pathogens. 106th General Meeting of the American Society for Microbiology, Orlando, Florida, May 21-25, 2006.
  18. Ruifang Xu, Robert D. Stedtfeld, Erdogan Gulari, James M. Tiedje, and Syed A. Hashsham. Influence of Target Preparation on Microarray Based Community Analysis of Indicator Organisms. 106th General Meeting of the American Society for Microbiology, Orlando, Florida, May 21-25, 2006.
  19. Sarah M. Miller, Alok Dhawan, Robert D. Stedtfeld, Dieter M. Tourlousse, James M. Tiedje, and Syed A. Hashsham. Real-Time Hybridization Behavior in a Flow-Through DNA Biochip as a Function of Temperature, Flow Rate, and Time. 106th General Meeting of the American Society for Microbiology, Orlando, Florida, May 21-25, 2006.
  20. Vidya Srinivasan, Alok Dhawan, Robert D Stedtfeld, Sarah M Miller, Trinh Pham, Jean Marie Rouillard, James M Tiedje, Erdogan Gulari, and Syed A Hashsham. Detection of Pathogens in Environmental Samples with Gold Nanoparticles and Silver Enhancement on Microarray Using a Flatbed Scanner. 106th General Meeting of the American Society for Microbiology, Orlando, Florida, May 21-25, 2006.
  21. Yu Yang, Robert D. Stedtfeld, Jean Marie Rouillard, Erdogan Gulari, James M. Tiedje, Syed A. Hashsham. Synthetic virulence and marker gene targets as surrogates for validation of pathogen biochips. 106th General Meeting of the American Society for Microbiology, Orlando, Florida, May 21-25, 2006.
  22. Ahsan Munir, Sarah M Miller, Alok Dhawan, Robert D Stedtfeld, Jean Marie Rouillard, Erdogan Gulari, James M Tiedje, and Syed A Hashsham. Hybridization Behavior of Vancomycin Resistance Genes in a Flow-Through DNA Biochip using a Computational Approach. 106th General Meeting of the American Society for Microbiology, Orlando, Florida, May 21-25, 2006.
  23. S.A. Hashsham, L. M. Wick, R. Stedtfeld, D. Tourlousse, S. Baushke, J-M. Rouillard, J. Klappenbach, J. R. Cole1, E. Gulari, J. M. Tiedje. DNA Biochips for Diagnostics, Source Tracking, and Microbial Community Analysis: Challenges and Selected Solutions, 105th ASM General Meeting, Atlanta, Georgia, May 2005.
  24. S. Baushke, S. Kravchenko, L. Wick, R. Stedtfeld, D. Tourlousse, A. Herzog, T. Huynh, J-M. Rouillard, E. Gulari, J.M. Tiedje, and S.A. Hashsham. Statistical Analysis of DNA Microarray Data for Parallel Microbial Detection and Mixed Community Fingerprinting, 105th ASM General Meeting, Atlanta, Georgia, May 2005.

  25. Herzog, A, R. Stedtfeld, T. Huynh, S. Baushke, J. Weiss, J-M. Rouillard, E. Gulari, J. Tiedje, and S.A. Hashsham. Challenges in Developing Community Fingerprinting Oligonucleotide Microarrays using the 16S rRNA Gene, 105th ASM General Meeting, Atlanta, Georgia, May 2005.

  26. Elizabeth Kruszewski, Y. Matsumura, V. Denef, L. Wick, K. Yadav, R. Xu, S. Baushke, R. Stedtfeld, . J. Tiedje and S. Hashsham. Detection of Vancomycin Resistant Enterococcus faecalis (N00-410) and Enterococcus faecium (N97-330) Using Microarrays, 105th ASM General Meeting, Atlanta, Georgia, May 2005.

  27. R. D. Stedtfeld, L. M. Wick, S. W. Baushke, J.M. Rouillard, X. Yongmei, J. Cole, J. Klappenbach, E. Gulari, J. M. Tiedje, S. A. Hashsham. Validation Strategy for a DNA Biochip: Comparison of Synthetic Targets and Extracted DNA Fragments for 16S rDNA Probes, 105th ASM General Meeting, Atlanta, Georgia, May 2005.

  28. Ruifang Xu, R. Stedtfeld, J-M. Rouillard, E. Gulari, J. Tiedje, and S.A. Hashsham. Evaluation of Labeling Protocols for Enhanced Sensitivity of In Situ Synthesized Biochips, 105th ASM General Meeting, Atlanta, Georgia, May 2005.

  29. Tourlousse, D., Herzog, A., Baushke, S., Tiedje, J.M., Hashsham, S.A. Development and validation of a DNA chip for the detection of virulence genes of human pathogens. 105th ASM General Meeting, Atlanta, Georgia, May 2005.

  30. Hashsham, S.A. and J.M. Tiedje. DNA biochips for diagnostics, source tracking, and microbial community analyses: issues and selected solutions. To be presented at the 10th International Symposium on Microbial Ecology, Microbial Planet: Subsurface to Space, Cancun, Mexico, August 22nd -27th, 2004.
  31. Denef, V.J., Park, J., Wick, L., Vervaeren, H., Sys, K., Matsumura, Y., Goris, J., Tsoi, T., Verstraete, W., J.M. Tiedje and S. A. Hashsham. Use of highly flexible, in situ synthesized microarrays for differential gene expression, comparative genomics and environmental microbial detection. Genomes 2004: International Conference on the Analysis of Microbial and Other Genomes, Cambridge, United Kingdom, April 14-17, 2004.
  32. Weiss, J., Wick, L., Yang, Y., Hashsham, S., Whittam, T., J.M. Tiedje, DNA microarrays as parallel microbial detection tools for water biosafety. ASM Biodefense Research Meeting, Baltimore, Maryland, March 7-10, 2004.
  33. Weiss, J., Denef, V., Klappenbach, J., Cole, J., Gulari, E., Rouillard, J.-M., Whittam, T., Rose, J., Hashsham, S., and J.M. Tiedje. Microarrays for the parallel detection and identification of microbes indicative of fecal matter contamination in water. To be presented at the 10th International Symposium on Microbial Ecology, Microbial Planet: Subsurface to Space, Cancun, Mexico. August 22nd -27th, 2004.
  34. Tourlousse, D., Weiss, J., Stedtfeld, R., J.M. Tiedje and S. Hashsham. Development of a DNA chip targeting virulence genes of human pathogens. To be presented at the 10th International Symposium on Microbial Ecology, Microbial Planet: Subsurface to Space, Cancun, Mexico. August 22nd -27th, 2004.
  35. Stedtfeld, R.D., Weiss, J., Tourlousse, D., J.M. Tiedje, and S.A. Hashsham. Innovative validation strategy for optimizing DNA biochips: comparison of synthetic targets and real DNA fragments. To be presented at the 10th International Symposium on Microbial Ecology, Microbial Planet: Subsurface to Space, Cancun, Mexico. August 22nd -27th, 2004.
  36. Wick, L.M., Whittam, T.S., Gulari, E., J.M. Tiedje and S.A. Hashsham. Development of in situ synthesized oligonucleotide microarrays (XeoChips) as parallel detection tools for water biosafety. To be presented at the 10th International Symposium on Microbial Ecology, Microbial Planet: Subsurface to Space, Cancun, Mexico. August 22nd -27th, 2004.
  37. Denef, V.J., Patrauchan, M.A., Florizone, C.M., Park, J., Tsoi, T.V. , Hashsham, S.A., Verstraete, W., Eltis, L.D., J. M. Tiedje. A transcriptomic, proteomic and genetic investigation of cellular processes involved in biphenyl and benzoate metabolism by Burkholderia xenovorans LB400. To be presented at the 10th International Symposium on Microbial Ecology, Microbial Planet: Subsurface to Space, Cancun, Mexico. August 22nd -27th, 2004.
  38. S. Dobbelaere, N. Boon, H. Vervaeren, J. Matthys, K. Sys V. Denef, S. Hashsham and W. Verstraete. Development and validation of a microarray for the identification of microorganisms responsible for bulking and foaming in WWTPs. European Symposium on Environmental Biotechnology, Oostende, Belgium, 25-28 April 2004.
  39. Hashsham, S., J-M. Rouillard, X. Gao, S. Callister, J. Cole, V. Denef, T. Tsoi, J. Wibbenmeyer, E. Gulari, and J. Tiedje. Highly parallel microbial detection using in situ synthesized flexible biochips. 103rd General Meeting of the American Society for Microbiology to be held in Washington D.C., May 18-22, 2003.
  40. Denef, V.J., T.V. Tsoi, J. Park, J.-M. Rouillard, H. Zhang, X. Gao, J. Wibbenmeyer, S.A. Hashsham, J.M. Tiedje. Outlining genome-wide carbon source specific metabolic networks in  Burkholderia sp. nov. LB400. 103rd General Meeting of the American Society for Microbiology, Washington D.C., May 18-22, 2003.
  41. Jenkins, TM, SA Hashsham, JR Cole, and JB Rose. Virulence-factor activity relationships (VFARs) database for waterborne pathogens. ASM the 103rd General Meeting of the American Society for Microbiology to be held in Washington D.C., May 18-22, 2003.
  42. Callister, S.J. and S.A. Hashsham, Modulus of stability and genome expression data. Poster to be presented at the “Environmental Genomics Workshop”, sponsors: University of Idaho and Idaho Engineering and Environmental Laboratory’s Biotechnology Department. Idaho Falls, Idaho, September 19-20, 2002.
  43. Callister, S. and S.A. Hashsham. Quantifying the relationship between genomic response and perturbation by modulus of stability. Proceedings of the 102nd General Meeting of the American Society for Microbiology. Salt Lake City, Utah, May 19-21.2002.
  44. Park, Joonhong, Tamara Tsoi, Syed A. Hashsham, Jim Cole, Stephen Callister, and James M. Tiedje. Design of specific nucleotide probes for genomic microarrays that explore gene expression patterns in a polychlorobiphenyl-degrading bacterium. “Bioremediation and biodegradation: current advances in reducing toxicity, exposure and environmental consequences”, Asilomar, CA. (poster presentation), June 9-12, 2002.
  45. Callister, Stephen, Joonhong Park, Tamara Tsoi, James Cole, James Tiedje, and Syed Hashsham. Examining DNA-DNA hybridization between chlorinated compounds oxidizing species using a combination PCR/oligo microarray. “Bioremediation and biodegradation: current advances in reducing toxicity, exposure and environmental consequences”, Asilomar, CA, June 9-12, 2002.
  46. Denef, Vincent, Bergholz, P., Joonhong Park, Syed Hashsham, Tamara Tsoi, James Tiedje. Assessing the sensitivity of oligonucleotide microarrays when studying bioaugmented bacterial communities in the environment. “Bioremediation and biodegradation: current advances in reducing toxicity, exposure and environmental consequences”, Asilomar, CA., June 9-12, 2002.
  47. Musarrat, J., J. Larabee, C. Criddle, and S.A. Hashsham, Monitoring the abundance of mRNA transcripts associated with carbon tetrachloride dechlorination in Pseudomonas stutzeri strain KC under different environmental conditions using DNA microarray. Proceedings of the 101st General Meeting of the American Society for Microbiology. Orlando, Florida, May 20-24, 2001.
  48. Denef, V.J., C.A. Kachel, J.L.M. Rodrigues, S.A. Hashsham, T.V. Tsoi, and J.M.Tiedje. DNA microarray approach to monitor engineered mixed PCB-degrading communities. Proceedings of the 101st General Meeting of the American Society for Microbiology. Orlando, Florida, May 20-24, 2001.
  49. Callister, S.J., H.L. Ayala, and S.A. Hashsham. Quantification of Pseudomonas cepacia G4 in a dense mixed microbial community using a dual laser microarray scanner. Proceedings of the 101st General Meeting of the American Society for Microbiology. Orlando, Florida, May 20-24, 2001.
  50. Dollhopf, S.L., S.A. Hashsham, and J.M. Tiedje. Interspecies interactions affecting spirochete species in methanogenic environments. Proceedings of the 101st General Meeting of the American Society for Microbiology. Orlando, Florida, May 20-24, 2001.
  51. Hashsham, S.A., J. Musarrat, M. Tijdens, V. Denef, T. Tsoi, C.S. Criddle, and J.M. Tiedje. Development of DNA microarray technology for mixed microbial communities. Proceedings of the 9th International Society for Microbial Ecology, Amsterdom, Netherlands, August 27-September 1, 2001.
  52. Dollhopf, S.L., S.A. Hashsham, and J.M. Tiedje. Application of neural networks in microbial community analysis: Detecting patterns and making predictions. Proceedings of the 9th International Society for Microbial Ecology, Amsterdom, Netherlands, August 27-September 1, 2001.
  53. Hashsham, S.A., S, Dollhopf, F.B. Dazzo, and J.M. Tiedje, Molecular data and mathematical modeling in biological wastewater treatment. Proceedings of the NSF Sponsored U.S.-Egypt Workshop in Microbial Ecology, National Research Center, Cairo, Egypt. May 6-11, 2001.
  54. Dollhopf, S.L., S.A. Hashsham, A. Fernandez, F.B. Dazzo, and J.M. Tiedje. Microbial community structure as a function of hydraulic retention time length and number in lab-scale methanogenic chemostats.  COE Symposium on the Evaluation of Advanced Waster Water Treatment Technology Systems using Functions of Complex Microbial Communities. Tokyo, Japan, 2000.
  55. Dollhopf, S.L., S.A. Hashsham, A. Fernandez, F.B. Dazzo, and J.M. Tiedje. Spirochete populations in methanogenic chemostats: Population dynamics and ecophysiology VAAM Microbiology 2000 Meeting. Munich, Germany. 2000.
  56. Dollhopf, S., S.A. Hashsham, F. Dazzo, C. Criddle, R. Hickey, and J. Tiedje. Community succession in methanogenic digesters to spirochete dominated communities Proceedings of the 99th General Meeting of the American Society for Microbiology. Chicago, Illinois, May 27-June 02, 1999.
  57. Hashsham, S., A. Fernandez, S. Dollhopf,  F. Dazzo, R. Hickey,  J. Tiedje,  and C. Criddle. Modeling microbial ecosystems: implications of molecular techniques. Proceedings of the 99th General Meeting of the American Society for Microbiology. Chicago, Illinois, May 27-June 02, 1999.
  58. Hashsham S., T. Marsh, A. Fernandez, L. Raskin, S. Seston, F. Dazzo, R. Hickey, C. Criddle, and J. Tiedje.    Microbial ecology of anaerobic digesters: stability and persistence during shock loads. Proceedings of the 8th International Symposium on Microbial Ecology. Halifax, Canada, August 9-14, 1998.
  59. Fernandez A., S. Huang, S. Hashsham, R. Hickey, C. Criddle, and J. Tiedje. Dynamics of bacterial populations and functional stability in perturbed anaerobic reactors. Proceedings of the 8th International Symposium on Microbial Ecology. Halifax, Canada, August 9-14, 1998.
  60. Dollhopf S.L., S. Hashsham, F. Dazzo, C. Criddle, R. Hickey, and J. Tiedje. Novel community structure in a model methanogenic system: implications for carbon and energy flow. Proceedings of the 8th International Symposium on Microbial Ecology. Halifax, Canada, August 9-14, 1998.
  61. Hashsham S., T. Marsh, S. Seston, F. Dazzo, R. Hickey, C. Criddle, and J. Tiedje. Perturbed anaerobic microbial communities: structure and stability. Proceedings of the 98th General Meeting of the American Society for Microbiology. Atlanta, Georgia, May 14-19, 1998.
  62. Seston, S., Hashsham, S., Dazzo, F., Criddle, C., Hickey, R, and Tiedje, J. Comparison of spirochete populations in three anaerobic reactors using ARDRA and cultivation techniques. Midwest Molecular Microbial Ecology Meeting 1998. Michigan State University, East Lansing, MI, 1998.
  63. Seston, S., Fernandez, A., Hashsham, S., Criddle, C., Hickey, R., and Tiedje, J. Community change during stable reactor function: A phylogenetic analysis. Midwest Molecular Microbial Ecology Meeting 1997. University of Wisconsin-Milwaukee, Milwaukee, WI, 1997.
  64. Fernandez A.T., J. Xing, S. Hashsham, C. Criddle, and R. Hickey. Diversity among glucose fermenters in methanogenic reactors. Proceedings of the 97th General Meeting of the American Society for Microbiology. Miami, Florida, May 19-23, 1997.
  65. Hashsham, S.A., and D.L. Freedman. Enhanced biotransformation of carbon tetrachloride by Acetobacterium woodii using hydroxocobalamin. Proceedings of the 96th General Meeting of the American Society for Microbiology, New Orleans, LA, May 19-23, 1996.
  66. Hashsham, S. and D. L. Freedman.  Enhanced biotransformation of carbon tetrachloride under methanogenic conditions,” The University of Notre Dame Hazardous Waste Conference, Notre Dame, IN, (August 31-September 4).,1992