$1.7 Million Grant Supports Harvesting of Alternative Fuel from Bacteria

June 2, 2010

R. Mark Worden, MSU professor of chemical engineering, is part of a group receiving $1.7 million from the U.S. Department of Energy Advanced Research Projects Agency-Energy to build a reactor system for Ralstonia eutropha, a bacterium that scientists aim to engineer to metabolize hydrogen and carbon dioxide to produce isobutanol, a fuel that can be used as a replacement for gasoline.

Anthony Sinskey, professor of biology at Massachusetts Institute of Technology, leads the genetic engineering team.

“The MIT group is focused on the biology of the bacterium and engineering it to produce isobutanol,” Worden explained. “Early this spring, they realized they needed a bioreactor expert, and I was asked to be a collaborator. My role is to build a reactor system at MSU for this unique fermentation system.”

Worden has to overcome two major challenges in developing the bioreactor system. Once Ralstonia eutropha is engineered to produce isobutanol, the isobutanol eventually will build up to a toxic level and kill the bacterium. So he must create a way to “harvest” the isobutanol without interrupting the fermentation process.

Secondly, because the bacterium’s energy source – hydrogen gas – is not very water-soluble, Worden must figure out how to feed it to the bacterium in a water-based system.

“We also have to make sure the bioreactor system is safe,” Worden said. “The Ralstonia cells need a little bit of oxygen to grow, but hydrogen and oxygen together are flammable. So I’ll figure out how the two gases can co-exist in the system and minimize any risk of explosion.”

Worden plans to start building the bioreactor immediately.

To read the entire news release, go to http://news.msu.edu/story/7907/